摘要
利用^(15)N稀释法研究小麦接种肺炎克雷伯氏菌(Klebsiella pneumoniae)43菌株的联合固氮作用。在小麦种子萌动后播种于土盆中,用43菌株接种,定苗后每盆施入^(15)N丰度为30%的标记硫酸铵25mg,培育70d后测定根、茎的干物质重、含氮量和^(15)N%原子超,并用乙炔还原法测定根系存在的固氮酶活性。结果表明,小麦根系存在固氮有机体,直接为植株提供氮素占其总氮量的15.3—22.1%,干物质重和^(15)N%含量与对照相比差异显著。
The technique of ^(35)N isotope dilution was used to verify that nitrogen was fixed by Klebsiella pneumoniae 43 strain in association with triticum aestivum. Germinated seeds grow siol pots were inoculated with K. pneumoniae 43. (NH_4)_2SO_4 enriched with 30% ^(15)N was used 25mg/pot. After 10 weeks the shoots and roots were analyzed separately for dry matter, N content, total N, and atom% ^(15)N excess. The acetylene reduction technique was used to test in the presence of N-fixing organism on the roots. The data of ^(15)N isotope dilution demonstrated that up to 15.3—22.1% of nitrogen from T. Aestivum were derived from associative N fixation and Klebsiella pneumoniae. The dry matter yield and atom% ^(15)N excess of wheat significant were increased but were not significant effect on N content and total N inoculated with K. pneumoniae.
出处
《微生物学报》
CAS
CSCD
北大核心
1989年第3期200-203,共4页
Acta Microbiologica Sinica
关键词
固氮作用
肺炎克氏菌
小麦
Klebsieila pneumoniae
Triticum aestivum
Nitrogen fixation
