摘要
所筛的41株属于根霉属的菌侏,均具有从葡萄糖产生延胡索酸的能力。其中,R25产延胡索酸能力最高,同时产生部分L-苹果酸。该菌株被鉴定为无根根霉(Rhizopus arrhizus)。普通变形杆菌(Proteus vulgaris)P1具有强的延胡索酸酶活性,可将延胡索酸转化为L-苹果酸。无根根霉R25在含有12%葡萄糖的培养基中,31℃振荡培养3天,进行延胡索酸发酵。然后接入在液体培养基中31℃生长2天的普通变形杆菌P1。在同样条件下发酵2天,L-苹果酸产量可达5.48g/100ml。在含有18%的薯干粉培养基中发酵,能得到相同的产量。发酵产物经分离提纯为白色结晶,经纸色谱、红外光谱分析和熔点测定,与已知的L-苹果酸一致。
During the screeing of 41 strains of the genus Rhizopus, all strains of Rhizopus were found to be capable of producing fumaric acid from glucose. Among these, the strain R25 which showed the highest yield of fumaric acid accompanied with some L-malic acid, was identified as Rhizopus arrhizus. Proteus unlgaris Pl exhibited a high fumarase activity to convert fumaric acid to L-malic acid.
When R. arrhizus R25 was shake-cultured to produce fumaric acid at 31℃ for 3 days in the medium containing 12% of glucose, and then associated with P. uulgaris Pl grown at 31℃ for 3 days in liquid medium, after 2 days, L-malic acid yields was 5.48 g/100 ml. Similar yields of L-malic acid were obtained in the medium containing 18% sweet potato mash. The product isolated and purified from broth was white crystal. The R_f value, infrared adsorption spectrum and meking point of this crystal coincided with those of the authentic L-malic acid.
出处
《微生物学报》
CAS
CSCD
北大核心
1989年第2期129-136,共8页
Acta Microbiologica Sinica
