摘要
在紫外光诱导硫氧还蛋白还原酶(TrxR)产生荧光光谱的实验基础上,对TrxR溶液的谱线特性及其产生机理进行了研究.实验结果表明,在波长为253.7 nm的紫外光激励下,TrxR溶液能够产生较强的荧光,其光谱覆盖了280～720 nm的范围,谱峰形状主要由两个宽阔的峰及分布在宽峰上的诸多细锐的次峰组成;随着TrxR溶液浓度的改变,宽峰和锐峰分别表现出不同的变化趋势.根据分子内部能量转换理论以及共振拉曼散射理论,对TrxR产生的荧光光谱的机理进行了理论分析.理论和实验研究结果均表明中心波长位于336 nm的宽峰是来自于TrxR分子中的色氨酸的荧光峰,而诸多锐峰则是TrxR分子的共振拉曼散射.对波长为253.7 nm的紫外光激励的TrxR溶液荧光光谱的研究有助于研究溶液状态下TrxR分子的构像、结构以及分子的电子振动态等.
Based on the experiment of fluorescence spectra of thioredoxin reductase (TrxR) from human brain by the excitation of ultraviolet light (UV-light), this paper studies the spectral characteristics and their origins. The experimental results show that, under the excitation of UV-light, TrxR solution emits spectra of region from 280 nm to 720 nm, including two wider bands and many sharper peaks. The wider bands and the sharper peaks represent different tendency along with the change of solution concentration. According to the theory of conversion of internal energy and resonant Raman scattering, the theoretical analyses of TrxR fluorescence spectra are educed. The results indicate that the wide band with the peak locating at 336 nm is the fluorescence from tryptophan in TrxR and the sharper peaks mostly come from the resonant Raman scattering of TrxR. These researches on the emission spectra of TrxR solution may represent an effort to better understand the conformation, structure and vibration of TrxR molecule.
出处
《中国激光》
EI
CAS
CSCD
北大核心
2005年第4期576-580,共5页
Chinese Journal of Lasers
基金
高等学校优秀青年教师教学科研奖励计划项目(2003 2008)资助。
