摘要
目的:观察Aβ1-15疫苗免疫接种成年恒河猴的特异性体液免疫应答。方法:5只雄性成年恒河猴分别在0、2、6、10、14、18、2 2周肌肉注射法接种Aβ1-15疫苗;ELISA法检测恒河猴血清抗Aβ1-42 抗体及其IgG亚型的水平;Westernblot法检测血清Aβ1-42 抗体的特异性;免疫组化方法观察抗血清与Tg2 5 76转基因鼠脑Aβ斑的识别。结果:疫苗接种后第8周,恒河猴血清中出现明显的抗Aβ1-42 抗体,抗体滴度随接种次数的增加而升高,第2 4周达1∶384 0 ,疫苗停止接种后抗体水平开始下降;血清抗Aβ1-42 IgG以IgG1为主;血清Aβ1-42 抗体具有高度特异性;抗血清可识别结合Tg2 5 76转基因鼠脑组织的Aβ斑。结论:Aβ1-15疫苗可有效诱导成年恒河猴产生特异性体液免疫应答。
AIM: To observe the humoral immune response in adult rhesus monkey induced by Aβ 1-15 vaccine. METHODS: 5 adult male rhesus monkeys were injected intramuscularly with Aβ 1-15 vac cine at baseline and at week 2, 6, 10, 14, 18, 22. The titer and IgG isotypes of the antibody against Aβ 1-42 in the serum were measured with ELISA. The specificity of the antibody against Aβ 1-42 was determined by Wester n blotting. The Aβ plaques in Tg2576 transgenic mouse brain were stained with t he antisera using immunohistochemistry method. RESULTS: At the eighth week after the vaccination, antibody against Aβ 1-42 bega n to develop significantly i n the serum. The titers of the antibody increased following vaccine boosted and reached 1: 3 840 at the twenty-fourth week, then decreased after the terminat ion o f inocunation. The IgG1 was accounted for the highest level in the antisera pool . The antibody against Aβ 1-42 showed high specificity. The Aβ plaques in Tg2576 transgenic mouse brain were labeled with the antisera. CONCLUSION: Aβ 1-15 vacci ne could induce vigorously specific humoral immune responses in adult rhesus mon key.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第4期761-764,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目 (No .30 4 0 0 5 12 )
广东省自然科学基金重大项目 (No.2 0 0 13137)
广东省自然科学基金资助项目 (No .0 4 30 0 2 18)
广州市科技局项目 (No .2 0 0 4Z3-E0 15 1)
中山市科技局重点攻关项目 (No.4 2 0 96 0 6 )
关键词
阿尔茨海默病
淀粉样肽
疫苗
恒河猴
Alzheimer's disease
Amyloid peptide
Vac cine
Macaca mulatta
