摘要
目的比较抗人球蛋白凝胶微柱分析和传统试管法检测O型孕妇血清中血型免疫性抗体的滴度差异及方法的灵敏度。方法用抗人球蛋白凝胶微柱分析和传统试管法平行检测53例与丈夫ABO血型不合的O型孕妇血清IgG抗A(B)水平,经配对t检验,比较两种方法检测结果的差异。结果凝胶微柱分析及试管法检测的平均抗体滴度分别是1:160和1:45,前者比后者高3.56倍,两种方法之间的差异有非常显著性意义(P<0.001);但两种方法的检测结果有相关性(r=0.829,P<0.001)。另外,以凝胶微柱分析检测398例O型孕妇,滴度>1:64的比例显著高于以试管法检测的文献报道结果。结论凝胶微柱分析比试管法具有更高的灵敏度,且实验操作简便,快速,更适合于实验室内的广泛应用;但因灵敏度过高,需对其决定值进行界定。
Objective To compare gel microcolumn assay and conventional tube test for IgG anti-A (B) titration. Methods Fifty-three blood samples of “O” group pregnant women, whose husbands were of non “O” group, were detected simultaneously by the conventional tube test and gel microcolumn assay. The results were analyzed using paired “t ” test. Results IgG anti-A (B) titration in gel microcolumn assay showed significantly higher titres (mean 3.56-fold) than that in conventional tube test in all samples studied (P < 0.001). The coefficient of correlation between the two methods for IgG anti-A (B) was 0.829 (P < 0.001). In addition, IgG anti-A (B) titres in 398 cases of “O” group pregnant women by gel microcolumn assay were significantly higher proportion above 1:64 than that by conventional tube test. Conclusion The gel microcolumn assay is superior to the conventional tube test. It has the further advantage of standardization of the test, shorter incubation time, and no washing procedure. The critical titre for anti-A (B) should be established in view of the high sensitivity of the gel microcolumn assay.
出处
《罕少疾病杂志》
2005年第2期9-11,共3页
Journal of Rare and Uncommon Diseases
关键词
血型免疫性抗体
抗人球蛋白试验
凝胶微柱分析
IgG antibodies for blood group antigens
Coombs’test
gel microcolumn assay
