摘要
目的 研究Aurora A高表达对人食管癌细胞的影响,以了解其在人食管癌侵袭转移中的作用。方法 采用细胞转染的方法,利用Aurora A基础水平表达较低的食管癌细胞系构建Aurora A高表达细胞株,比较其与转染空载的细胞,分析二者在表型差别。结果 Aurora A高表达细胞表现出明显的增殖优势,在体外细胞移动实验中表现出穿膜能力大大增强。结论 提示Aurora A高表达在人食管癌侵袭转移中有重要的意义。
Objective To observe the effect of Aurora-A on the esophageal cancer cell lines in order to analyze its role in malignancy of the development of human esophageal cancer.Method We developed the of Aurora-A over-expressing cell lines by transfecting this gene into the esophageal cancer cells that had low basal level of Aurora-A and then investigated the phenotypic alterations of these cell lines.Result With increased expression of the Aurora-A protein, the cells exhibit faster proliferation and stronger motility ability.Conclusions Aurora-A may contribute to invasive growth and metastasis of human esophagus cancer by promoting cell proliferating and motility.
出处
《河南肿瘤学杂志》
2005年第2期77-79,F002,共4页
Henan Journal of Oncology
基金
国家 973重大基础研究项目 (2 0 0 2CB51 31 0 1 )
