摘要
目的:探讨丙型肝炎病毒(hepatitisCvirus,HCV)抑制T细胞IL-2分泌的机制,为预防、诊断和治疗丙型肝炎提供新思路。方法:利用卟啉醇肉豆蔻酸乙酸酯(phorobol12-myristate13-acetate,PMA)、植物血凝素(phyto-haemagglutinin,PHA)、Ca2+以及抗CD3抗体(OrthoKungTcell3,OKT3)、抗CD81单克隆抗体以及HCV表面蛋白(envelopeproteinofHCV,EP)刺激jurkatT细胞,采用ELISA方法检测培养液中IL-2的产生量并讨论。结果:单独采用PMA和PHA,PHA和Ca2+,OKT3和抗CD81单克隆抗体刺激jurkatT细胞24h,会产生大量的IL-2。如果在采用上述刺激之前,分别使用抗CD81单克隆抗体或EP预处理jurkatT细胞1h,则在上述刺激之后IL-2的产量明显减少。结论:由于EP可与CD81分子特异结合,可以说明HCVEP通过T细胞表面的CD81分子抑制了IL-2的分泌,导致免疫功能受损。
Objective: To study the mechanism of hepatitis C viyus ( HCV) inhibit IL-2 secretion of human T cells, and try to find a new way to prevent, diagnose and treat the hepatitis caused by HCV. Methods: Phorobol 12-myris-tate 13-acetate (PMA) , phytohaemagglutinin (PHA) , Ca2+ (ionomycin) , anti-CD3 monoclonal antibody (Ortho Kung T cell 3, OKT3) , anti-CD81 monoclonal antibody and envelope protein (EP) of HCV were used to activate jurkat T cells. IL-2 was measured by ELISA. And the results were discussed. Results: Using PMA&PHA, PHA&ionomycin, OKT3&anti-CD81 antibody to activate jurkat T cells separately for 24h will produce a lot of IL-2. But if pretreat the cells by anti-CD81 antibody or EP for 1h, the production of IL-2 will be decreased observably. Conclusion: Because EP can bind with CD81 molecule specifically, it is well known that HCV can inhibit IL-2 production of T cell by the binding of EP and CD81, to induce the hurt of immune response.
出处
《沈阳医学院学报》
2005年第1期8-10,共3页
Journal of Shenyang Medical College
