摘要
通过适当的样品处理方法，游离的和结合的植物雌激素［大豆素（ｄａｉｄｚｅｉｎ），雌马酚（ｅｇｕｏｌ），染料木素（ｇｅｎｉｔｅｉｎ），芒柄花素（ｆｏｉｍｏｎｏｎｅｔｉｎ），香豆雌酚（ｃｏｕｍｅｓｔｒｏｌ）和美皂异黄酮（ｂｉｏｃｈａｎｉｎＡ）］被从新鲜植物材料的提取物中分离出来。并在不同的紫外光波长下，可被ＨＰＬＣ法定量测定。根据滞留时间和标准品的添加，而鉴别出植物雌激素的层析波峰。本方法的测定灵敏度为２ｐｐｍ。白三叶草（Ｔｒｉｆｏｌｉｕｍｒｅｐｅｎｓ）样品的加样回收率在８０％－１００％之间（平均回收率变异系数为５．４％）。通过比较游离植物雌激素的含量测定，本方法的提取率比经典的甲醇浸提法更为有效。
By using a suitable sample preparation,free and bound phytoestrogens (daidzein,equol,genistein,formononetin,coumestrol and biochanin A) can be isolated from extracts of fresh plant material and subsequently quantified by HPLC at different wave Iengths.Chromatographic peaks assigned to phytoestrogens were identified by retention time and by comparison to the addi-tion of standards.The method is sensitive to about 2 ppm of phytoestrogens.Recoveries of phytoestrogens in spiked samples of white clover(Trifolium repens L.)ranged between 80% and 100 %(average 5.4 % R. S.D).This method also showed more efficient extraction for some phytoestrogens than the traditional soaking method by comparison with their free phytoestrogen concentrations.
出处
《云南植物研究》
CSCD
1994年第4期424-430,共7页
Acta Botanica Yunnanica
