摘要
目的观察镇痛剂量吗啡鞘内泵入对大鼠细胞免疫功能的影响。方法40只雄性SD大鼠随机分为5组(n=8):假手术组(F组)、生理盐水组(NS组)、M1、M2、M3组(吗啡泵入速率分别为25、5、10μg·h-1)。除F组外各组采用改良Yaksh法进行鞘内置管,置管后5d采用Alzet泵持续泵人生理盐水或吗啡,共7d。第7天各组大鼠进行福尔马林炎性疼痛实验,根据痛级(PIS)评分评价吗啡镇痛效应,处死大鼠后计算睥脏指数并分离脾脏单个核细胞进行培养,甲基-3H胸腺嘧啶核苷掺入法检测脾T淋巴细胞增殖水平,乳酸脱氢酶释放法检测自然杀伤细胞活性。结果与NS组比较,MI、M2、M3组在福尔马林炎性疼痛第一时相和第二时相的PIS评分值、脾脏指数、脾脏T淋巴细胞增殖转化水平和NK细胞活性降低(P<0.05或0.01);与M1组比较,M2、M3组脾脏T淋巴细胞增殖转化水平及NK细胞活性降低(P<0.05);与M2组比较,M3组脾脏NK细胞活性降低(P<0.05)。结论镇痛剂量吗啡鞘内泵入可抑制大鼠细胞免疫功能,且泵入速率越大,免疫抑制效应越明显。
Objective To investigate the effects of intrathecal morphine on cell-mediated immunity. Methods Forty male SD rats weighing 250-300 g were randomly divided into 5 groups ( n = 8 in each group) : sham-operated group (F); saline group (NS) and 3 morphine groups (M1, M2, M3). The animals were anesthetized with intraperitoneal chloral hydrate 300-350 mg·kg-1 . Microspinal catheter was inserted into the subarachnoid space at the lumbar region according to modified Yaksh technique. Correct implantation of the spinal catheter was confirmed by aspiration of CSF. In the morphine groups, after 5 days morphine was continuously infused through the spinal catheter at 2.5 (Ml), 5.0 (M2) and 10 μg·h-1(M3) for 7 days. In NS group normal saline was continuously infused instead of morphine. On the 7th day 5% formalin 50 fd was injected into the plantar surface of left hindpaw. The number of flinches, lickings and total time of licking were recorded for 60 min. Pain intensity scoring (PIS) (0-3, 0 = no pain, 3 = severe pain) was used to assess the antinociceptive effect of intrathecal morphine. The animals were killed after evaluation of pain intensity. Body weight and spleen weight were measured. Spleen index (spleen weight/body weight) was calculated. T-lymphocyte function was evaluated based on Concanavalin-A (Con A) induced splenocyte proliferation. Modified lactic acid dehydrogenase (LDH) release assay was used to assess NK cell activity. Results The PIS scores were significantly lower in group M1 , M2 and M3 than in F and NS group. The spleen index, splenocyte proliferation induced by Con A and NK cell activity were significantly suppressed in the 3 morphine groups Conclusion Intrathecal morphine has significant antinociceptive effect and suppresses T-lymphocyte proliferation and NK cell activity in a dose-dependent manner
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2005年第2期118-121,共4页
Chinese Journal of Anesthesiology
基金
国家自然科学基金资助(30271256)
