摘要
采用共培养和脂质体介导两种方法将金黄地鼠附睾内的精子与 NFκB luc+ 质粒共孵育后,再与成熟的卵母细胞进行体外受精,用PCR、Southern blot及荧光原位杂交(FISH)等技术检测外源基因在精子细胞和在受精卵中的存在及存在部位,并对相关数据进行统计学比较分析。结果表明,金黄地鼠附睾内的活精子具有主动捕获外源基因的能力,而且外源基因只存在于精子细胞的头部,而且通过受精将外源基因带入了卵细胞,脂质体的存在增加了外源基因转染精子的效率和转基因效率。
Golden hamster spermatozoa and NF κ B-luc^+ were in cubated in co-culture using liposome mediated methods, and then were treated with oocytes by IVF. Two groups of samples were detected by PCR, Southern Blot and FISH. Analyzed the relative data by statistics. The results showed that spermatozoa of Golden hamster epididymis could take the foreign DNA voluntarily, and the foreign DNA existed in the head of the spermatozoa only and could enter into zygotes by IVF. The exist of liposome can improve the foreign gene transfect ratio and transgenic ratio.
出处
《遗传》
CAS
CSCD
北大核心
2005年第2期195-200,共6页
Hereditas（Beijing)
基金
广东省自然科学基金博士启动项目(No.04300440)
关键词
精子
转基因载体
脂质体
spermatozoa
transgenic vector
liposome
