摘要
①目的建立糖尿病并发感染大鼠模型,检测胰腺组织肿瘤坏死因子(TNFα) mRNA和蛋白表达的变化,评估糖尿病并发感染时的炎症改变.②方法采用链佐菌脲霉素(STZ)大鼠尾静脉注射法,诱发糖尿病模型;多细菌感染-腹膜炎模型参考盲肠结扎穿刺术(CLP)方法建立;糖尿病并发感染模型的建立是在STZ诱发血糖升高的基础上行CLP术.大鼠分为正常组、手术对照组、糖尿病组、感染组和糖尿病并发感染组.ELISA方法测定C反应蛋白(CRP)、真胰岛素和TNFα蛋白表达的水平;逆转录聚合酶链反应(RT-PCR)检测TNFα mRNA的半定量值.③结果将糖尿病和多细菌感染的动物模型结合,得到了满意的糖尿病并发感染的模型.糖尿病并发感染组大鼠TNFα mRNA的半定量分析结果和循环中TNFα水平与正常组、手术对照组和糖尿病组比较明显升高(H=7.56,q=3.25～5.26,P<0.05;F=9.49,q=3.76～12.92,P<0.05、0.01),与单纯感染组之间无明显差异(P>0.05);而胰腺组织中TNFα蛋白水平在糖尿病并发感染组较其他各组明显升高(F=7.78,q=2.80～4.32,P<0.05).④结论糖尿病并发感染时扩大了炎症反应,TNFα水平可能反映了胰腺损伤程度.
Objective To establish a rat model of diabetes mellitus(DM) complicated with infection, detect the changes of tumor necrosis factor α(TNFα) mRNA and protein in pancreas, and assess the inflammatory changes in this condition. Methods The rat model with diabetes mellitus complicated with infection was obtained by STZ-injection and CLP-operation. The rats were divided into normal, sham-operated, DM, infection, and DM complicated with infection(DMI) groups. The C-reactive protein(CRP), insulin and TNFα were measured by ELISA; semi-quantitative TNFα mRNA in pancreas was detected by RT-PCR. Results The rat models were established successfully. The levels of TNFα mRNA in pancreas and TNFα of plasma were higher than those in the normal, sham-operated and DM groups (H=7.56,q=3.25-5.26, P<0.05;F=9.49, q=3.76-12.92,P<0.05, 0.01),but not significantly different with those in the infection group (P>0.05). The concentration of TNFα in the pancreas increased significantly in the DMI group (F=7.78,q=2.80-4.32,P<0.05). Conclusion DM complicated with infection extended the inflammation reaction. The level of TNFαmay reflect the pancreatic injuries.
出处
《青岛大学医学院学报》
CAS
2005年第1期61-63,66,共4页
Acta Academiae Medicinae Qingdao Universitatis
关键词
糖尿病
感染
大鼠
肿瘤坏死因子Α
diabetes mellitus
infection
rats
tumor necrosis factor α
