摘要
建立反相液相色谱法测定大鼠血浆中胡黄连苷Ⅰ(HD-Ⅰ)的含量,并对胡黄连苷-Ⅰ在大鼠体内动力学特征进行分析研究。以芍药苷为内标物,血浆样品经Vc抗氧化,乙腈沉蛋白后,采用AgilentXDBC18色谱柱(250mm×4.6mm,5μm)分离,流动相:乙腈-水,梯度洗脱程序[(0-1)min]20:80,[(1-2)min]24:76,[(2-6)min]24:76,[(6-8)min]20:80,([(8-14)]min20:80;检测波长:277nm。结果:HD-Ⅰ线性范围(0.1-150)μg/mL(r=0.9994),最低检测浓度25ng,回收率分别为(94.70±3.27)％、(95.09±0.78)％、(95.91±0.83)％,日内、日问精密度准确度均小于10％。大鼠单次静脉注射HD-Ⅰ7mg/kg、14mg/kg、40mg/kg后,血药浓度-时间曲线均呈二室开放模型,半衰期T1/2β分别为(27.07±6.56)min、(29.78±6.24)min、(31.41±4.75)min,清除速率CL(s)值分别为(0.050±0.017),(0.049±0.012),(0.046±0.025)mg·kg-1·min-1(μg·mL-1),平均滞留时间MRT平均为(15.86±0.29)min,曲线下面积AUC和AUC(so)与剂量呈正相关。证实方法准确、灵敏度高,可用于HD-Ⅰ体内过程的研究。HD-Ⅰ在大鼠体内符台线性动力学二室开放模型,代谢快,分布广,清除速率快。
To establish a RP-HPLC method for the analysis of pharmacokinetics on Picroside-Ⅰ in Rats,the plasma samples which had been added Vc in it were diluted by acetonitrile to deposit protein. The agilent XDB C18 column (250 mm×4.6 mm,5 μm)was used as analytical column under room temperature. The mobile phase was a mixture of acetonitrile-water at a flow rate of 1.0 mL·min-1. The UV detection wavelength was 277 nm. It is resultsed that the calibration curve was linear in the range of (0.1-150) μg·mL-1(r=0.9994). The lowest detectable concentration of Picroside Ⅰ was 25 ng·mL-1(S/N=3.4). The recovery of the methods were more than 90%, RSD of intra-day and inter-day were less than 10% respectively. After three injection dose of Picroside-Ⅰ, the concentration-time curves administration were both confirmed to two-compartment open models in rats. It is conclused that the concentration-time curves administration were both confirmed to two-compartment open models. The method is accurate and reliable. It can be used for the pharmacokinetic studies of Picroside Ⅰ in rats plasma. The sharmacokinetics characteristics showed Picroside Ⅰ was metabolized rapidly in the rat, distr ibuted abroad, and eliminated soon.
出处
《科学技术与工程》
2005年第7期431-434,共4页
Science Technology and Engineering
