摘要
目的研究白介素(IL)-13在急、慢性荨麻疹患者中的作用。方法对22例急性荨麻疹患者和20例慢性荨麻疹患者,采用流式细胞仪检测淋巴细胞内IL-13、IL-4、干扰素酌(IFN-酌);用ELISA法测定血清IL-13和总IgE,并与正常人对照组进行比较。结果经流式细胞仪检查发现,急性荨麻疹患者淋巴细胞IL-13的表达显著升高,与正常人对照组差异有统计学意义;而慢性荨麻疹患者的IL-13表达略有升高,与正常人对照组差异无统计学意义;IL-4的表达在两组患者中差异均无统计学意义。ELISA检测血清IL-13的结果与流式细胞仪检测结果相符;急慢性荨麻疹患者IL-4的表达与正常人对照组差异无统计学意义;IFN-酌的表达在荨麻疹患者中无明显改变。IgE的表达在急、慢性患者中均显著升高。但IL-13的表达和IgE抗体的量无相关性。结论IL-13在荨麻疹的发病中发挥了重要作用,尤其是急性荨麻疹。
Objective To investigate the role of IL-13 in the patients with acute and chronic urticaria. Methods In 22 patients with acute urticaria, 20 patients with chronic urticaria and 19 normal controls, the levels of IL-13, IL-4 and IFN-γ of peripheral T lymphocytes were measured by flow cytometry. The serum concentrations of IL-13 and total IgE were tested by ELISA. Results The results of flow cytometry showed that the level of IL-13 of the patients with acute urticaria was significantly higher than that of the normal controls (P < 0.05). Although the expression level of IL-13 also increased in the patients with chronic urticaria, there was no significant difference compared with that of the normal controls. There was no significant difference in the expiession level of IL-4 between the acute and chronic urticaria patients. The IL-13 results measured by ELISA were consistent with those by flow cytometry. There were no changes in the expression of IFN-γ in these patients. The total serum IgE level of patients with acute and chronic urticaria was significantly higher than that of normal controls (P < 0.01). Conclusion IL-13 may play an important role in the pathogenesis of urticaria, especially in acute urticaria.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2005年第3期163-164,共2页
Chinese Journal of Dermatology
