摘要
目的 :探讨四氢生物蝶呤 (BH4)对肾缺血再灌注损伤 (IR)模型的作用及其作用机制。方法 :复制大鼠肾缺血再灌注模型 ,手术前 1h喂饲BH4,动态检测其对肾组织NO含量、cNOS和iNOS活性的影响 ,并通过肾功能、MPO、MDA的变化来评价BH4对肾IR模型的作用。结果 :BH4能够升高再灌注早期cNOS活性 ,增加内皮源性NO含量 ,减轻炎症反应 ,减弱再灌注后期对iNOS的诱导作用。结论 :BH4可改善再灌注初期cNOS活性 ,保护肾功能。
AIM: To investigate the effects and mechanisms of tetrahydrobiopterin (BH 4) on renal ischemia reperfusion injury in rats. METHODS: The ischemia reperfusion model was induced and 20 mg·kg -1 BH 4 was orally administrated 1 h before the operation. NO, NOS activity, MPO activity and MDA and renal function were measured in order to assess the efficacy of BH 4. RESULTS: BH 4 ameliorated cNOS during the early period of reperfusion and increased the NO level in renal tissue which diminish inflammation responses and decrease the induction of iNOS. CONCLUSION: BH 4 can attenuate renal ischemia reperfusion injury, and it may be related to ameliorating cNOS during the early period of reperfusion.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2005年第2期172-175,共4页
Chinese Journal of Clinical Pharmacology and Therapeutics
