摘要
目的 :了解不同压力CO2 气腹对慢性肾功能衰竭模型大鼠肾功能衰竭的影响 ,并探讨CO2 气腹造成肾功能损害的可能机制。方法 :建立 5/ 6肾切除大鼠慢性肾功能衰竭模型 ,分别施加 0 67kPa(5mmHg)、1 3 3kPa(10mmHg)CO2 气腹压 ,检测解除气腹即时、1d、4d血肌苷 (Scr)、尿素氮 (BUN)水平及肾组织匀浆超氧化物歧化酶 (SOD)活力、丙二醛 (MDA)含量变化。结果 :解除气腹即时Scr开始升高 ,1d时升高显著 ,第 4d时下降接近对照组水平 ;BUN变化不明显。肾组织SOD和MDA水平也分别在解除气腹即时开始下降和上升 ,气腹后第 1d变化较明显 ,至第 4d时渐趋回复 ;与对照组相比 ,1 3 3kPa气腹压各组造成的变化具有显著意义。结论 :CO2 气腹可引起慢性肾功能衰竭致肾脏功能改变 ,可能与气腹压力的直接压迫使肾皮质缺血及解除气腹后血流再灌注产生的自由基损害有关。
Objective:To explore the effect of carbon dioxide(C O 2 )pneumoperitoneum with different pressure on renal function in a rat model of ch ronic renal failure and its possible mechanism.Methods:Rats With 5/6 renal ablation were used as animal models.They were divided into 0 67kPa(5m mHg)group and 1 33kPa(10mmHg)group.The control group was performed shamed-opera tion.The level of serum creatine(Scr)and blood urine nitrogen(BUN),activity of s uperoxide dismutase(SOD)and content of malonyldialdehyde(MDA)were measured as so on as CO 2 was deflated,one day later and four days later,respectively. Results:Scr level began to increase as soon as CO 2 was deflated.One da y later it increased significantly but four days later it decreased near to that i n control group.BUN level had no remarkable change.When the rats were free from CO 2 pneumoperitoneum,SOD content began to decrease and MDA began to increase.O ne day later,these changes were comparatively remarkable.Four days later,they gr adually returned to normal.Compared with control group,changes in 1 33 kPa(10mm Hg)group had much significance.Conclusion:CO 2 pneumoperitoneu m can result in reversible changes in renal function.Its mechanism might be rela ted to ischemia in renal cortex caused by direct compression of pneumoperitoneum and injury of free radical caused by ischemia-reperfusion after gas deflation.P neumoperitoneum with high pressure has more significant influence on renal funct ion.
出处
《西南国防医药》
CAS
2004年第1期1-4,共4页
Medical Journal of National Defending Forces in Southwest China
关键词
解除
损害
水平
直接
目的
产生
活力
artificial pneumoperitoneum,renal failure,chronic,SOD,M DA.
