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RACE技术及其在寄生虫全长cDNA克隆中的应用 被引量：3

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摘要 PCR技术的出现大大提高了传统基因分离和克隆效率。以PCR为基础的RACE(rapidam plificationofcDNAends)技术是一种简单、敏感且有效扩增cDNA的 5′和 3′端未知序列区域的方法。RACE技术被广泛应用于克隆全长cDNA 。
作者 韩海勃 曹建平
机构地区 中国疾病预防控制中心寄生虫病预防控制所寄生虫病原和媒介生物学重点实验室
出处 《国外医学（寄生虫病分册）》 2005年第1期14-18,共5页 Parasitic Diseases Foreign Medical Sciences
关键词 全长CDNA克隆 寄生虫 扩增 PCR技术 未知序列 基因克隆 敏感 RACE技术 克隆效率 基因分离
分类号 R373 [医药卫生—病原生物学] Q78 [生物学—分子生物学]
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参考文献29

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同被引文献39

  • 1刘博,苏乔,汤敏谦,袁晓东,安利佳.应用于染色体步移的PCR扩增技术的研究进展[J].遗传,2006,28(5):587-595. 被引量：33
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  • 5Liu X W, Gorovsky M A. 1993. Mapping the 5' and 3' ends of Tetrahymena thermophila mRNAs using RNA |igase mediated amplification of cDNA ends (RLM-RACE). Nucleic Acids Res, 21 : 4954 -4960.
  • 6Maruyama I N, Rakow T L, Maruyama H I. 1995. cRACE: A simple method for identification of the 5' end of mRNAs. Nucleic Acids Res, 23 (18) : 3796 -3797.
  • 7Wu Wei-Ming, Tsai Hsiang-Ju, Jong-Hwei S Pang, Hsin-Shin Wang, Hong-Shang Hong, Yun-Shien Lee. 2005. Touchdown thermocycling program enables a robustsingle nucleotide polymorphism typing method based on allele-specific real-time polymerase chain reaction. Analytical Biochemistry, 339:290-296.
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国外医学（寄生虫病分册）
2005年 第1期

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