摘要
以解淀粉芽孢杆菌（Ｂａｃｉｌｌｕｓａｍｙｌｏｌｉｑｅｆａｃｉｅｎｓ）Ａ－４为出发菌株，经诱变处理，选育多重抗药性突变株。研究结果表明，抗药性突变株α－淀粉酶产量提高的正变率和正变幅度明显大于非抗药性突变株，负变率和负变幅度也较高。经ＮＴＧ和ＮＴＧ－ＵＶ诱变处理，获得一株林可霉素、Ｄ－环丝氨酸和万古霉素多重抗性突变株ＬＣＶ＿５（Ｌｉｎ￣ｒ，Ｃｓ￣ｒ，Ｖｍ￣ｒ），其α－淀粉酶活力比出发菌株Ａ－４提高３２．５％，发酵周期短，摇瓶为４０ｈ，酶活力达４６１ｕ／ｍｌ．
In this study,the mutagensis of NTG and NTG-UV to the Bacillus amyloliqefa-ciens A- 4 is studied.Lincomycin-resistant mutants(Lin￣r),D-cycloserine-resistant mutants(Cs￣r ),Vancomycin-resistant(Vm￣r)were isolated after treatment with NTG and NTG-UV.The production of α- amylase from the resistant mutants were obviously higher thenthat of no-resistant mutants.We have screened the high productivity strain of which theactivity of α- amylase about was raised 32.5%。The α-amylase activity reached about 461u/ml after 40 hr of shaking culture.
关键词
Α-淀粉酶
高产菌体
选育
酶制剂
Higher productivity strains of α-amylase
NTG
D-cycloserine
Vanconlycin
Lincomycin
