摘要
目的 将尿液质粒缺口 连接酶链反应 酶联免疫吸附法 (Gap LCR ELISA)用于检测重庆地区孕妇以了解围产期沙眼衣原体 (chlamydiatrachomatis ,CT)的感染现状。 方法 以 5 12名孕妇首段尿(firstvoidurine ,FVU )及配对宫颈上皮细胞为研究对象 ,按照扩大金标准 ,比较质粒探针和外膜蛋白探针Gap LCR ELISA法用于不同类型临床标本检测CT感染的方法学指标。 结果 (1)孕妇的CT感染呈现很强的隐匿性 ,由扩大金标准所确证的CT真阳性共 42例 ,所检重庆地区围产期孕妇的CT感染率为 8.2 % (4 2 / 5 12 ) ,其中 88.1% (3 7/ 42 )可同时从尿道和生殖道检出CT ,而另 9.5 % (4 / 42 )和 2 .4% (1/ 42 )仅能单独分别从生殖道或尿道检出。 (2 )Gap LCR ELISA用质粒与外膜蛋白作为探针检测FVU中CT的灵敏度分别为 90 .48%和 71.43 % (P <0 .0 5 ) ,质粒Gap LCR ELISA用于宫颈上皮细胞和FVU两种标本的灵敏度分别为 97.62 %和 90 .48% (P >0 .0 5 ) ,特异度均为 10 0 %。 结论 质粒Gap LCR ELISA法用于孕妇FVU以检测围产期无症状CT感染患者 ,是一种非侵袭性的、高度灵敏特异的、适于发展中国家和地区进行大规模流行病学调查的方法。
Objective To study the prevalence of perinatal chlamydia trachomatis infection in the city of Chongqing. Methods First void urine (FVU) samples and cervical smear from 512 pregnant women (gestational age≥28 weeks) were collected. According to the “expanded gold standard”, the methodologic indices were compared between Gap LCR ELISA using plasmid probes and omp1 probes on FVU and cervical smear, respectively. Results (1) CT infection in pregnancy is usually asymptomatic. Forty two CT positive cases were confirmed by “expanded gold standard” indicating a prevalence of CT infection in pregnant women in Chongqing was 8.20%(42/512). Among these 42 women, 37 (88.1%) were detected from both FVU and cervical smear, while positive results were shown in 4 cases (9.5%) from cervical smear and only one case (1/42) 2.4% from FVU . (2) The sensitivity of Gap LCR ELISA using plasmid probes and ompl probes were 90.48% and 71.43% ( P <0.05) in detecting CT in FVU. Meanwhile in detecting CT in cervical smear and FVU samples, the sensitivity were 97.62% and 90.48% with Gap LCR ELISA using plasmid probes ( P >0.05). The specificity of all Gap LCR ELISA tests were 100%. Conclusions FVU plasmid Gap LCR ELISA is a noninvasive, highly sensitive and specific method which is suitable for large scale screening for perinatal CT infections in pregnant women in developing countries and regions.
出处
《中华围产医学杂志》
CAS
2005年第1期38-42,共5页
Chinese Journal of Perinatal Medicine
基金
国家自然科学基金资助 (基金编号 :30 2 0 0 30 0 )
关键词
孕妇
CT感染
质粒
ELISA法
围产期
尿液
沙眼衣原体感染
结论
不同类型
灵敏
Chlamydia trachomatis
Chlamydiaceae infections
Ligase chain reaction
Enzyme-linked immunosorbent assay
Pregnancy complications, infectious
