摘要
Ｂ（ａ）Ｐ和ＣＴＰ是煤焦化作业人群中职业性肺癌发病率与死亡率增高的重要因素。本研究应用体外细胞转化模型，观察了微量元素硒（Ｓｅ）对Ｂ（ａ）Ｐ和ＣＴＰ诱发人胚肺细胞癌前变化的保护作用。结果表明，人胚肺细胞分别经终浓度Ｂ（ａ）Ｐ和ＣＴＰ为１．０μｇ／ｍｌ处理之前３０分钟经Ｓｅ终浓度为１．０×１０－５Ｍ／ｍｌ预先保护后，分别进行体外传代至第１２～１３代时，出现形态学转化特征，与Ｓｅ防护组比较，转化程度减轻，或不发生转化，染色体损伤减轻，其防护效率分别为６２．９±１３．７％和５８．０±１７．０％，提示Ｓｅ对Ｂ（ａ）Ｐ和ＣＴＰ具有较好的防癌效果。
Benzopyrene (B(a)P) and coal-tar pitch (CTP) are one of the important factors of enhancing incidence and mortality in the taroperating population with professional lung cancer.The transformation system of cell in vitro was used in this studies. The per-carcinogenic changes of the human embryo lung cell (HEL-9201) in vitro exposed to final concentration of lμg/ml B (a) P or CTP, respectively , and protective effect of Se (1×10-5mol/ml) on cell transformation were observed. The results shown that the characteristics of morphological transformation of HEL-9201 cells were exhibited after treatment 11th to 12th passages by B (a)P or CTP, for contract with Se treated group. The Se protective group cells havn't the characteristics of transformation, it was reduced for chromosome injury. The protective efficiency of Se on B(a)P and CTP were 62.9% and 58.0% , respectively. The results suggest that the Se is better an agent to prevent earcinogenesis.
出处
《卫生毒理学杂志》
CSCD
1994年第1期3-6,共4页
Journal of Health Toxicology
关键词
硒
苯并芘
沥青
转化
防护作用
Selenium
Benzopyrene
Coal-tar Pitch
Transformation
Protective effect.
