摘要
目的:观察在成软骨诱导培养条件下,细胞传代对骨髓间充质干细胞(MSCs)体外成软骨能力的影响。方法:不同代MSCs成软骨诱导后,观察细胞生物学特性以及通过免疫荧光、RT-PCR测定特异性软骨细胞外基质aggrecan的表达情况。结果:经成软骨诱导后,第2、4代MSCs表达aggrecan明显较第6、8代细胞高。结论:MSCs很可能由多种形态功能接近,分化潜能有略有差异的细胞组成;在成软骨诱导培养条件下,对此传代后成软骨能力减弱。
Objective:In vitro chondrogenic potential of serially passaged rabbit bone marrow derived mesenchymal stem cells (MSCs) was evaluated for the feasibility of repairing cartilage defect with engineered cartilage. Methods: Chondrogenic potential of MSCs in vitro was evaluated by means of investigating cartilage-specific extracellular matrix aggrecan with RT-PCR, Western blot and immunofluorecence. Results: Aggrecan expressed less in the chondrogensic primary MSCs than in the P2,4 MSCs,least in the P6-8 MSCs. Conclusion: Although chondrogenic markers aggrecan were prominent in the less passaged BMCs continuously cultured with chondrogenic supplements,they decreased with passaging;MSCs maybe are consisted of many mesenchymal cells with similar appearance and function,being a little difference in differentiating different kinds of tissue cells;Establishment of the culture conditions that permit the rapid expansion of BMCs while retaining their potential for chondrogenic differentiation will be required for clinical repairing cartilage defect.
出处
《中国临床医学》
北大核心
2005年第1期104-107,共4页
Chinese Journal of Clinical Medicine
基金
国家自然科学基金资助项目(30271307)
