摘要
应用直接免疫荧光（ＤＩＦ）技术及抗Ｃ３－酶联免疫吸附试验（ＥＩＴＳＡ）分别检测了２５例硬红斑（ＥＩ）皮损血管壁上组织沉积免疫复合物（ＴＩＣ）和血清中循环免疫复合物（ＣＩＣ），结果显示：（１）ＥＩ血管壁上存在着ＩｇＧ、ＩｇＭ及ＩｇＣ，其总阳率为８４％（２１／２５），且ＩｇＭ、ＩｇＧ－ＴＩＣ为主（７２％，５２％），ＴＩＣ有两种方式，（２）８４％ＥＩ血清ＣＩＣ阳性，且以ＩｇＭ、ＩｇＧ－ＣＩＣ为主（７６％，５６％），ＩｇＭ－ＣＩＣ与ＩｇＧ－ＴＩＣ、ＩｇＧ－ＣＩＣ与ＩｇＧ－ＴＩＣ之间呈显著性一致，治疗后ＩｇＭ、ＩｇＧ－ＣＩＣ明显降低。上述结果首次证实ＥＩ为ＩＣ型血管炎，ＴＩＣ来自ＣＩＣ，Ⅲ型变态反应参与了ＥＩ的发病机理，动态观察ＥＩ血清ＣＩＣ可以做为ＥＩ的发病机理，动态观察ＥＩ血清ＣＩＣ可以做为ＥＩ治疗效果评估的指标。
In this paper,we detected the tissue-deposited immune complexes (TIC )in the lesions and circulating immune complexes (CIC) in sera of erythema induratum (EI) using direct immunofluorescence (DIF) AND ANTI-C3 enzyme linked immunosorbent assay (ELISA). The results were as follows : (1)three kinds of TIC,IGA.IgM-TIC . were deposited in the walls of blood vessels in 84% of EI and mainly IgG,IgM-TIC (76%, 56% ). There were two kinds of deposited ways. (2) three CICs were 84% positive and mainly IgG, IgM-CIC. and significantly reduced (P 0. 01 ) after treatment. There was a significant relativity between IgG-CIC and IgG-TIC, IgMCICand IgM-TIC. It indicates that EI is a kind of immune complexes type vasculitis,and type Ⅲ hypersensitivity plays an important role in the pathogenesis of EI,TICs mainly come from CICs. The detection of CICs may serve as an assessment of clinical therapeutic effect of patients with EI.
出处
《泰山医学院学报》
1994年第1期68-74,共7页
Journal of Taishan Medical College
关键词
硬红斑
免疫复合物
直接免疫荧光
erythema induratum (EI)
immune complexes (IC)
tissue-deposited immune complexes (TIC)
circulating immune ccomplexe (CIC)
direct immunofluorescence (DIF)
enzyme linked immunosorbent assay (ELISA).
