摘要
采用核团微量注射、推挽灌流结合行为测痛，神经肽的放免分析等实验方法分别从突触后受体和突触前递质释放的角度探讨了网状旁巨细胞核（ＲＰＧＬ）中的内阿片肽与针刺镇痛的关系。实验结果显示：大鼠ＲＰＧＬ微量注射纳洛酮（５μｇ／０．５μｌ／２ｍｉｎ）可部分翻转针刺镇痛效应，与电针加生理盐水组比较，注药后５ｍｉｎ、１０ｍｉｎ差异均有显著性意义（Ｐ＜０．０５）。ＲＰＧＬ内纳洛酮翻转针效的作用具有一定的剂量—效应关系。用放免测定的方法观察ＲＰＧＬ灌流液中内阿片肽含量在针刺前后的改变，显示电针组动物电针２０ｍｉｎ后，动物ＲＰＧＬ灌流液中亮氨酸脑啡肽（ＬＥＫ）的释放显著高于对照组（Ｐ＜０．０５）。其中高针效组动物电针后ＲＰＧＬ灌流液中β—内啡肽（β—ＥＰ）的释放高于对照组（Ｐ＜０．０５），而低针效组动物电针后β—ＥＰ的释放无明显增多。动物电针后的痛阈升高与ＲＰＧＬ中ＬＥＫ及β—ＥＰ的释放增多呈正相关（ｒ＝０．５２９，Ｐ＜０．０５；ｒ＝０．４８３，Ｐ＜０．０５）；电针２０ｍｉｎ后动物ＲＰＧＬ灌流液中强啡肽Ａ^（１～１３）（ＤｙｎＡ^（１～１３））的释放虽有所增加，但与对照组相比，无显著性意义（Ｐ＞０．０５）。上述结果分别从突触后受体?
The present study was to investigate the role of endogenous opioid peptides of rat's nucleus reticularis paragigantocellulari lateralis(RFGL) in acupuncture analgesia (AA) using techniques of brain microinjection, push-pull perfusion and radioimmunoassay (RIA). The results showed that the effect, of AA was partially reversed by microinjecting naloxone (5μg/0.5μl/2min) into the RPGL. Significant difference was found between naloxone and NS group (P>0. 05). The reversal effect of naloxone was dose-dependan by push-pull perfusion technique and RIA of neuro-peptides, it was found that after 20 minutes'electroacupuncture (EA), the release of LEK from the RPGL in the EA group was significantly higher than that in the control group (P<0. 05). The release of β-EP from the RPGL in the EA group with higher efficacy was significantly higher than that in the control group(P<0. 05). There was a positive correlation between the changes of release of LEK and β-EP and the increase of pain threshold. The release of DynA1￣13 tends to show a barely significant extent (P>0. 05 ).The results mentioned above imply that the activation of the endogenous opioid peptides within RPGL produce an advantageous effect on AA.
出处
《泰山医学院学报》
1994年第1期29-35,共7页
Journal of Taishan Medical College
关键词
微量注射
内啡肽
RPGL
纳洛酮
microinjetion
push - pull perfusion
radioimmunoassay
naloxone
leu-enkephalin
BB-endorphin
