摘要
本文研究了葡萄糖、蔗糖和甘油三种冷冻保护剂在不同浓度下对卤虫无节幼体的冷冻保护作用。研究表明用２０％甘油进行保护，无节幼体在－３０℃下耐受９－１０分钟，存活率高达８６．４％，对照组冷冻至－３０℃则全部死亡。本文还研究了浓度为２０％的甘油、ＤＭＳＯ和甲醇对日本绒螯蟹合浦亚种 状幼体的冷冻保护作用，结果表明用２０％甲醇保护的蚤状幼体当冷冻至－２４℃时存活率仍有７５．３％。
Chill tolerance of nauplii of Anemia spp. put into freezer at -30℃ for 30 minutes with and without the addition of glucose, sucrose and glycerol at various concentrations was determined. The average liquid cooling rate was 3-5℃ /min, and the average solid cooling rate was 1-1. 3℃/min. All of the control without the addition of the cryoprotective agent died, the addition of the cryoprotective agent enhances the cooling tolerance of nauplii, and the survival varied according to the kind of cryoprotective agent,concentration and balance time, the highest survival of nauplii reached 86. 4% in 20% glycerol when frozen to -30℃for 9-10 minutes. Chill tolerance of zoea of Eriocheir japonicus hepuensis at -25℃was also studied with 20% glycerol,DMSO and methanol. The average liquid cooling rate was 3-4℃ /min, the average solid cooling rate was 0. 3-0. 5℃ /min. All of the control died when frozen to -23℃, the best rsult was 75. 3% survived in 20% methanol when frozen to -24℃. Besides, the significance of these results for the development of cryogenic larval banking techniques was discussed.
出处
《水产学报》
CAS
CSCD
北大核心
1994年第4期321-325,共5页
Journal of Fisheries of China
关键词
冷冻保存
卤虫
日本绒螯蟹
合浦亚种
无节幼体
cryopreservation
cryoprotective agent
Anemia spp.
Eriocheir japonicus hepuensis
nauplii
zoea
