摘要
用计算机为工具对３２种生物钙调素（ＣａＭ）一级结构的保守性和变异性进行了数量分析，结果表明ＣａＭ分子高度保守，其保守性强于同类生物的细胞色素ｃ，脊椎动物ＣａＭ间的同源性在９８％以上，被子植物ＣａＭ间以及被子植物与脊椎动物ＣａＭ间的同源性高于８８％；脊椎动物与单细胞藻类ＣａＭ间的同源性也在８０％以上。对亲源关系较近的物种，它们的ＣａＭ主要免疫反应位点和靶酶结合位点间的同源性大于全序列间的同源性；对亲源关系较远的物种，它们的ＣａＭ主要免疫反应位点和靶酶结合位点间的差异大于它们全序列间的差异。
The conservatism and variability of camodulin (CaM) primary struCture of 32 kinds of organisms were analysed quantitatively by the aid of microcomputer. It was found that CaM was more conservative than cytochrome c in the same kinds of organisms. The similarities of complete sequence, were above 98% between every two kinds of vertebrate CaM, above 88%between every tWo kinds of angiosperm CaM or between angiosperm CaM and vertebrate CaM, above 80% between vertebrate CaM and unicellular green alga CaM. The similarities of main immunoreactive site and target enzyme-binding site between every two kinds of CaM,were greater than those of complete sequences among those close species in evolution, but smaller among those farther species.
出处
《生物物理学报》
CSCD
北大核心
1994年第4期543-551,共9页
Acta Biophysica Sinica
关键词
调钙蛋白
一级结构
保守性
变异性
数量分析
Camodulin Primary structure Conservatism Variability Quantitative analysis
