摘要
研究了ＤＮＡ与红细胞膜脂质过氧化产物的相互作用。与无ＤＮＡ的反应体系相比，ＤＮＡ的加入明显增加４４０ｎｍ处的荧光强度，这种效应是由于ＤＮＡ与过氧红细胞膜生成了新的荧光物质，其激发波长３１５ｎｍ，发射波长４１０ｎｍ．维生素Ｅ强烈抑制荧光产物的形成。ＤＮＡ经分离、纯化后用ＥＢ作荧光探针研究表明，ＤＮＡ二级结构发生了改变，ＤＮＡ—ＥＢ复合物荧光光谱，ＤＮＡ—ＥＢ复合物之间的能量转移效率均有明显变化。同时证实ＤＮＡ损伤具有碱基特异性，即主要是鸟嘌呤受到损伤。
Interaction of DNA with peroxidizing erythrocyte membrane was studied. Compated with the system without DNA, the addition of DNA obviously enhance the fluorescence intensity at 440nm.It is due to the formation of new fluorescence product between DNA and peroxidizing erythrocyte membrane, with maximum excitation wavelength at 315um and maximum emission wavelength at 410nm. Vitamin E strongly inhibital the formation of fluorescence product. After DNA was separated and purified, and EB was used as fluoascent probe to detect the higher structure of DNA we found that fluorescent spectrum of DNA-EB changed obviously, and the energy transfer effichacy from DNA tO EB decreased , this showed that DNA was damaged. Among the four usual bases, guanine damage was much more serious than those of the other three.
出处
《生物物理学报》
CAS
CSCD
北大核心
1994年第2期317-322,共6页
Acta Biophysica Sinica
基金
国家自然科学基金
关键词
红细胞膜
DNA
能量
转移
过氧化脂质
Erythrocyte membrane Lipid peroxidation DNA Energy transfer
