摘要
以天花粉蛋白（ＴＣＳ）晶体结构模型作为ＲＩＰｓ的代表，ＲＮＡ四核苷酸ＧＡＧＡ作为底物类似物，用计算机图象模拟建立了ＴＣＳ和ＧＡＧＡ相互作用的模型。利用这一模型解释了ＲＩＰｓ的Ｎ－糖苷酶活性的作用机制。这一模型对专一性识别也能解释，且与一些生化实验结果相符；对某些实变体的活性改变也能有相应的解释．
A model of substlate interaction with Trichosanthin, a RIP,, was established. Atetlaribenuclootide GAGA was chosen as the subehate analogue. Using this model, a possiblemechanism of N-glycosidase achvity of Race as well as the subetrate specificity wasinterpretal in agreement with some bincheemical resuults. The alteration in activity of somemutants could also be well explained.
出处
《生物物理学报》
CAS
CSCD
北大核心
1994年第1期13-18,共6页
Acta Biophysica Sinica
基金
香港大学及理工拨款委员会研究经费资助
