摘要
植物显性插入突变载体的构建陆建荣，金振华（中国科学院发育生物学研究所，北京１０００８０）关键词Ｔ－ＤＮＡ，显性插入突变，异位表达插入突变是遗传学研究的有效工具。插入的ＤＮＡ可以作为标记，使得分离有关基因变得较为快速方便。在植物分子生物学研究中，除了转...
出处
《生命的化学》
CAS
CSCD
1994年第6期49-50,共2页
Chemistry of Life
同被引文献42
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1KARDAILSKY I,SHUKLA V K,AHN J H,et al.Activation tagging of the floral inducer FT[J].Science,1999,286:1962-1964.
-
2ROSSO M G,LI Y,STRIZHOV N,et al.An Arabidopsis thaliana T-DNA mutagenized population (GABI-Kat) for flanking sequence tag-based reverse genetics[J].Plant Molecular Biology,2003,53(1-2):2 4 7-259.
-
3GROTEWOLD E.Plant functional genomics[M].Totowa,New Jersey:Humana Press,2003:177-188,345-364.
-
4OCHMAN H,GERBER A S,HARTL D L.Genetic application of an inverse polymerase chain reaction[J].Genetics,1988,120:621-623.
-
5LIU Y G,MITSUKAWA N,OOSUMI T,et al.Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junction by thermal asymmetric interlaced PCR[J].Plant Journal,1995,8(3):457-463.
-
6GREVE H D,NGUYEN K V,DEBOECK F,et al.T-DNA tagging of the translation factor eIF-4Al of Arabidopsis thaliana[J].PlantScience,2001,161:685-693.
-
7WEI W,TWELL D,LINDSEY K.A novel nucleic acid helicase gene identified by prooter trapping in Arabidopsis[J].Plant Journal,1997,11:1307-1314.
-
8WEIGEL D,AHN J H,BLAZQUEZ M A,et al.Activation tagging in Arabidopsis[J].Plant Physiology,2000,122:1003-1013.
-
9LUJR(陆建荣) JINZHH(金振华).Design of dominant insertional mutagenesis vector[J].生物工程进展,1994,14(5):34-35.
-
10HAYASHI H,CZAJA I,LUBENOW H,et al.Activation of a plant gene by T-DNA tagging:auxin-independent growth in vitro[J].Science,1992,258:1350-1353.
二级引证文献4
-
1王峰,黄璐圆.双元Ti载体的发展[J].西北植物学报,2006,26(11):2397-2401. 被引量：4
-
2侯雷平,王小菁,李洪清,李梅兰.应用Tail-PCR扩增蓝猪耳T-DNA侧翼序列[J].应用与环境生物学报,2009,15(6):871-874. 被引量：4
-
3杨诗怡,周小利,陈志芸,顾菁,廖菊够,陈穗云.植物基因功能验证技术概述[J].安徽农业科学,2017,45(34):136-140. 被引量：6
-
4韩梁彦,李树强,王丽丽,张春晓.一种产黄青霉突变体库的简单快速构建方法[J].基因组学与应用生物学,2019,38(12):5470-5474. 被引量：1
