摘要
本文研究１－甲基－４－苯基－１，２，３，６－四氢吡啶（ＭＰＴＰ）对体外培养大鼠中脑多巴胺（ＤＡ）神经元的毒性作用。取胚胎腹侧中脑，制成细胞悬液，常规体外培养。实验分为：正常对照组、高浓度ＭＰＴＰ（３０μｍｏｌ／ｍｌ）组、低浓度ＭＰＴＰ（３μｍｏｌ／ｍｌ）组和胆碱能神经元对照组。培养细胞定期抽出作免疫组化和荧光组化染色。高浓度ＭＰＴＰ组培养１０天，荧光组化阳性细胞已基本消失，此后酪氨酸羟化酶（ＴＨ）免疫反应的阳性细胞开始减少，至体外培养２周，每孔平均仅剩１４．５个，与正常对照组相比有显著性差异。残存的ＤＡ神经元突起缩短或消失。低浓度ＭＰＴＰ组培养２周后，大部分荧光组化阳性细胞消失，但对ＴＨ免疫反应阳性细胞的数量无明显影响。基底前脑胆碱能神经元体外生长不受ＭＰＴＰ干扰。实验结果提示ＭＰＴＰ对大鼠中脑ＤＡ神经元同样具有特异性损伤作用，其损伤程度与ＭＰＴＰ浓度有关。
The toxic effects of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to rat mesencephalic dopaminergic(DA) neurons were studied in vitro. The cell suspensions of ventral mesencephalon were obtained from embryo SD rat and were planted on 24 well culture plate. The experimental samples were divided into the normal control group, high dose MPTP group,low dose MPTP group and cholinergic neurons control group. The culture cells were examined using ABC immunocytochemistry and Faglu fluorescence histochemistry methods.In the high dose MPTP group,having been cultured for about ten days,the fluorescence histochemistry positive cells disappeared.After that,the tyrosine hydroxylase (TH)immunoreaction positive cells began to reduce. By the end of the second week, only 14.5 TH positive cells could be found averagely in each well.There were significant difference between high dose MPTP group and normal control group.The survival DA neurons had short or had no processes.In the low dose group,two weeks later,most of fluorescence histochemistry positive cells also disappeared,but the number of TH positive cells had no obvious change. The growth and development of basal forebrain cholinergic neurons was not disturbed by MPTP.The results indicated that MPTP toxicity for cultured mesencephalic DA neurons was dose-dependent,and that MPTP could specifically destroy the rat mesencephalic DA neurons.
出处
《南通医学院学报》
1994年第2期127-131,共5页
ACTA Academiae Medicinae Nantong
基金
国家自然科学基金
