摘要
从收集的５株枯草芽孢杆菌（Ｂａｃｉｌｌｕｓｓｕｂｔｉｌｉｓ）菌株中，筛选出一株具有一定产肌苷能力（１０ｇ／Ｌ），并带腺嘌呤缺陷型遗传标记的菌株．以该菌株为出发株，通过６０Ｃｏγ－射线和亚硝基胍复合诱变，得到一株对８－氮杂鸟嘌呤具有抗性，并且丧失了部分核苷磷酸化酶活性的突变株．在此基础上，通过正交试验，得到肌苷发酵最佳条件，在５Ｌ全自动发酵罐上进行了多次试验，肌苷产量达到１５ｇ／Ｌ．
The mutant HW-9 of Bacillus subtilis showing the property of Ade-,NP-,8-AGr, was selected from five strains by the methods of 60Co γ-ray and chemical NTG mutation.The optimal fermentation technology was got by orthgonal design.After four times of fermentation on five liter fermentor,the concentration of inosine in fermentation broth was about 15g/L,which was 31% more than that of original strain.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1994年第2期66-69,共4页
Journal of Nanjing Agricultural University
