摘要
本文报道作者于１９９２～１９９３年在澳大利亚应用ＤＥＡＥＳｅｐｈａｄｅｘＡ－５０层析法对腺病毒群特异性抗原─—六面体壳蛋白（Ｈｅｘｏｎ）进行提取、纯化并免疫动物获得高效价的腺病毒群特异性抗体。在抗原洗脱过程中分别在０．０５Ｍ、０．２Ｍ和０．４８Ｍ出现了３个波峰。壳蛋白的洗脱浓度为０．４８Ｍ。为明确其生物学特性，作者应用ＳＤＳ－ＰＡＧＥ电泳。证实其分子量大约为４５ＫＤ。本文的实验结果为解决腺病毒的诊断抗体提供了新的简便、经济的方法。
Using DEAE-Sephadex A-50 column, we suceessfully purified adenovirus group antigen-hexon. High titer antibodies were achieved by immunizing the animal with the purified antigen. In the process of chromatography, there were 3 peaks when the molarity of NaCl was 0.05M,0.20M and 0.48M,and hexon antigen was eluted at 0.48M,The SDS-PAGE showed the molecu lat we ight of hexon antigen was abeut 45KD.The study will hopely benifitpreparing diagnostic antibodies against adenoviruses.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1994年第4期252-254,共3页
Immunological Journal
基金
中国国际人才交流协会
