摘要
本实验应用杂交瘤技术，首次在国内建立了两株抗人补体终未阶段同源限制因子（ＨＲＦ）的杂交瘤细胞系（２Ａ７和１Ｆ１０）。经抗原特异性及功能分析提示它们所针对的抗体分子具有相似的功能，但抗原性和分子量不同：２Ａ７－Ａｇ为１８～２２ＫＤａ，１Ｆ１０－Ａｇ为６５ＫＤａ。进一步应用免疫荧光流式细胞分析、促同源补体溶血试验、中和实验、Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ、ＰＩ－ＰＬＣ处理等实验手段系统地鉴定了２Ａ７抗原的细胞分布、抗原特异性、分子特性及功能。表明２Ａ７与ＣＤ５９的命名抗体ＭＥＭ４３和１Ｆ５共同识别同一膜糖蛋白分子，该分子以糖脂链（ＧＰＩ）锚固于细胞膜上，证明２Ａ７为一株抗ＣＤ５９的单克隆抗体。
Two hybridomas.clone 2A7 and 1F10 were obtained by screening fOr antibcaly which caused hemolysisof human erythrocyte by human and EDTA-HuS. They react with two different proteins with MW of 18～22KDaand 65KDa respectively. Flow cytometric analysis showed that mAb 2A7 recognizes an antigen expressed on all peripher-al leucocyte and several cell lines.but was absent from U937 and Raji cell lines. This antigen was sensitive to treatmentwith pheephdylinosito-specific pheepholipase C(PI-PLC) on Jurkat cell line but not on erythtocyte. Compare mAb 2A7witb two known CD59 antibodies MEM4 3 and 1F5.it was found that three mAbe all recognized related epitopo on thesame erythrocyte glycoprotein. These results demostrated that 2A7 is a new monoclonal antithey against CD59.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1994年第4期218-223,共6页
Immunological Journal
基金
国家自然科学青年基金
"八五"全军青年基金
