摘要
本实验用３１Ｐ核磁共振谱仪，研究了短暂脑缺血对长期维持不同水平血糖的自发性糖尿病鼠脑内能量代谢、ｐＨ及再灌注后恢复的影响。结果发现，低血糖组（＜３ｍｍｏｌ／Ｌ）无机磷共振谱峰在缺血前高于其它各组，而ｐＨ的变化幅度最小。血糖为１３．７±１．２和２０．３±０．６ｍｍｏｌ／Ｌ的高血糖鼠，缺血引起的变化是相似的。缺血期间各组磷酸肌酸水平的下降无显著差别。脑组织ｐＨ的变化和高能磷酸化合物的水解，决定于缺血前的血糖水平。无机磷和ｐＨ的变化随着再灌注迅速恢复到缺血前的水平。然而，高血糖鼠磷酸肌酸水平并未完全恢复，其恢复程度决定于缺血前的血糖水平。本研究结果提示，正确地治疗糖尿病，可以减轻脑缺血引起的损害，这对于糖尿病人具有重要意义。
Using spontaneous diabetic BB rats, the effect of chronically maintained blood glucose levels on the degree of energy failure and the brain pH change during an ischemic insult, and on the subsequent recovery following reperfusion, has been studied with the use of in vivo 31P magnetic resonance spectroscopy. Prior to ischemia , hypoglycemic ( blood glucose < 3mmol/L)diabetic rats showed an increased inorganic phosphate peak intensity, with no significant pH change, as compared to other groups. During ischemia, the decrease in tissue pH and the hydrolysis of high energy phosphate metabolites were dependent on pre- ischemia blood glucose concentrations. Among hyperglycemic BB rats, similar ischemia- induced changes were found in subgroups with blood glucose levels of 13.7±1. 2 and 20.3±0. 6mmol/L, respectively, in keeping with the known dowm- regulation of glucose transport in diabetes. The decline in the phosphocreatine level during ischemia was not significantly different between groups. With reperfusion both inorganic phosphate and pH values rapidly to pre- ischemia values, Phosphocreatine levels, however, did not recover in hyperglycemic diabetic animals, with the degree of residual impairment dependent on the preischemia glucose level. The resuIts indicate that optimal management of diabetes may lessen the degree of injury within the ischemic penumbra, which is of greater size in diabetic patients.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1994年第1期22-25,共4页
Medical Journal of Chinese People's Liberation Army
关键词
糖尿病
脑缺血
能量代谢
NMR
Pmagnetic resonance spectroscopy
Diabetes
Cerebral ischemia
Energy metabolism
pH
