摘要
采用透射电镜观察了贝氏隐孢子虫在珍珠鸡气管和法氏囊的发育。贝氏隐孢子虫各期虫体均在上皮细胞微绒毛所形成的带虫空泡内发育，虫体基部有一营养器。滋养体呈圆形，有一个细胞核。胞质中有发达的粗面内质网。裂殖体经２或３次核分裂，以出芽方式形成４或８个裂殖子。成熟的裂殖子呈香蕉形，大小为２．８５×０．７０μｍ，被双层膜。小配子体由滋养体发育而来，内含多个缺少核仁的细胞核，细胞核移向胞质浅层，并进入胞质突起成为小配子的细胞核。大配子内可观察到二种成囊体和大量多糖颗粒，并在其胞质的空泡内发现小配子类似物。孢子生殖也在带虫空泡内进行，最终形成一个大残体和４个子孢子。子孢子有一个细胞核，富含微线和多糖颗粒。
Oocysts of Cryptosporidium baileyi isolated from ducks were orally inoculated into thirty guinea fowls to observe the development of this organism by the transmission electron microscope. All the life cycle stages of C. baileyi were observed attached to the surface of epithelial cells of the trachea and bursa of Fabricius, surrounded by a parasitophorous vacuole which appeared to be derived from microvilli of the host cell. The feeder organelle, through which the parasite acquires nourishment from host cell, was present in the base of parasite. The trophozoite was spherical, and possessed a nucleus and well-developed rough endoplasmic reticulum. The trophozoite developed into the schzont, in which four or eight merozoites were formed by budding in the parasitophorous vacuole following two or three nuclear divisions. The fully developed merozoite were banana-shaped, 2.85×0.70μm in size and covered with a double membrane pellicle. The development of the trophozoite into the microgametocyte was accompanied by nuclear division. Every nucleus which lackcd nucleoli migrated to the edge of the microgametocyte, and enter the protrusion of cytoplasm to become the nucleus of the microgamete. The microgametewas wedge-shaped and unflagellated, averaged 1.21×0.48μm in size. Two foims of wall fo rming body, numerous polysaccharides and microgamete-like structure located in a vacuole, were ob-served in the macrogamete. Sporogony took place in the parasitophorous vacuole, resulting in the formation of a residual body, and four sporozoites each of which had a nucleus, many micronemes and polyseccharide granules.
出处
《寄生虫与医学昆虫学报》
CAS
1994年第4期16-20,共5页
Acta Parasitologica et Medica Entomologica Sinica
基金
广东省青年基金
