摘要
小鼠经利福喷丁１０、２０、４０、８０或１２０ｍｇ·ｋｇ（－１）·ｄ（－１）ｐｏ。预处理１５ｄ后，采用光镜和透射电镜观察了小鼠肝脏组织和细胞超微结构的改变，并用酶组织化学法测定了１０种酶活性的改变。结果表明，利福喷丁剂量达到４０ｍｇ·ｋｇ（－１）以上时普遍出现肝浊肿变性，个别出现小叶结构破坏，细胞膜破损或局部点状坏死等组织学改变。此外线粒体肿胀明显，脂滴和溶酶体增多，内质网增生，个别区域狄氏间隙增宽、微绒毛减少，而核和胞膜基本正常，除ＡＮＡＥ和ＡｃＰ活性显著增高外，其余８种酶活性则显著下降。结果显示利福喷丁剂量大于４０ｍｇ·ｋｇ（－１）可明显损害小鼠肝脏。
After mice were pretreated with rifapentine (R773) 10, 20, 40, 80, or 120 mg. kg(-1). d(-1) po. for 15 days, the structure change of liver tissue and hepatoeyte was observed with light microscopy and electron microscopy. The activities of 10 enzymes in hepatocyte were also tested with eezpoe histochemical methods. When the pretreating dose of R773 was over 40 mg. kg(-1), the liver cloudy swelling degeneration was widespreadly occured. to some mice, the daniage of hepatic lobules, the rupture of cytomembrane, the local spotty necrosis and other histologic changes can also be observed.Besides, the results markedly showed the mitoehondrial swelling, the increase of fat droplet and lysosome, the broaden of Disse's spaces and the rarefaction of microvilli in some area. But the nucleus and cytomembraxie were normal on the whole. With the exception of significasit increase of ANAE and AcP activities, the activities of other tested enzymes were significantly decreased after the mice were pretreated with R773 40-120mg. kg(-1). d(-1) for 15 days.
出处
《华西医学》
CAS
北大核心
1994年第2期166-169,共4页
West China Medical Journal
关键词
利福喷丁
肝脏毒性
酶组织化学
Rifapentine
hepatotoxicity
enzyme histochemistry
ultrastructure
