摘要
通过诱导获得了移植的ＣＴＬ（细胞毒性Ｔ淋巴细胞）并为建立大规模培养系统而研究了其培养条件，通过外周血单核细胞（ＰＭＢＣ）与经Ｘ－射线辐射的肿瘤ＳＱ－５在含有白细胞介素（ＩＬ）１、２、４、６的Ｒｈａｍα培养液中共同培养３天以上，获得了抗人肺鳞状癌肿ＳＱ－５的人的ＣＴＬ．ＣＴＬ杀伤靶细胞的靶效比是１．在含有人的血清白蛋白（ＨＳＡ）培养液中这种ＣＴＬ生长很快，保存３个月以后仍保持很高杀伤活性，达９５％以上。与非靶的肿瘤细胞相比，ＣＴＬ表现出对靶细胞的特异活性，在一个月内即可获得足够量的ＣＴＬ（１０￣（１０）－１０￣（１１）个）．这些结果表明应用培养的ＣＴＬ治疗各种癌症是有良好前景的治疗技术。
Allogeneic cytotoxic T lymphocytes (CTL ) were induced, their culture condition wasinvestigated to established the lauge-scale culture system. Human CTL against human lungstluamous carcinoma SQ 5 were obtained by co-culturing peripheral blcod mononuclear cells(PBMCI on X- ray- irradiated confluent SQ - 5 for over 3 days in Rham a mediumsupplemented with interleukin (IL) 1, 2,4 and 6. CTL killed target cells within 24 hours at aneffectorltarget ratio 1. They grew up greatly in human serum albumin (HSA)containingmedium,and were maintained for over 3 months, keeping their killing activity at a significantlyhigh level ,above 95 %. CTL showed a specific activity to their target cells in comparison with non-target tum cells. An enough amount (10￣(10)-10￣(11) )of CTL can be obtained within one month.These results give a prospective therapy technique for various cancer,by using the cultured CTL.
出处
《华南师范大学学报（自然科学版）》
CAS
1994年第4期1-11,共11页
Journal of South China Normal University(Natural Science Edition)
关键词
CTL
诱导
细胞毒性
T淋巴细胞
肿瘤细胞
CTL
CTL induction
serum-free culture
killing activity
cytotoxicity
