摘要
谷氨酸氧化酶（ＧＯ）发酵液经（ＮＨ＿４）２ＳＯ＿４沉淀后用透析法获得酶粗品。利用ＧＯ测定谷氨酸发酵液中谷氨酸（ＧＬｕ）的含量与传统的瓦氏法结果一致，相关系数为０．９８６，相关方程为：ｙ＝０．９２８ｘ＋０．１２７（其中ｙ，ｘ分别表示氧化酶法与瓦氏法测定的值）。且这种方法比瓦氏法方便、简单、分析成本低、耗时少。利用ＧＯ法测定血清中谷草转氨酶（ＧＯＴ）和谷丙转氨酶（ＧＰＴ）的含量，与传统的试剂盒法比较，相关系数分别为０．９７８、０．９８４，相关方程为：ｙ＝０．９９８ｘ－０．１１０７，ｙ＝０．９５９ｘ＋０．０１３，其中ｙ为新方法测定值，ｘ是传统方法测定值。
Crude L-glutamate oxidase was obtained from liquid culture of streptomyces P- 26.The crude enzyme was used for the analysis of broth sample containing L-glutamate The result from our method was well correlated with that by Warburg method·Glutamate oxaloacetate transaminase and glutamate pyruvate transaminasc activities in serum were also determined by current method and with good correlation with conventional kit method.
出处
《华东理工大学学报（自然科学版）》
CAS
CSCD
1994年第5期611-614,共4页
Journal of East China University of Science and Technology
关键词
谷氨酸
谷氨酸氧化酶
谷草转氨酶
L- glutamate
L-glutamate oxidase
glutamate oxaloacetate transaminase
glutamate pyravate transaminase
