摘要
杂种犬11只,股动脉放血使平均动脉压为6.0kPa,90min后回输全部血液。维拉帕米组于休克30min时输入维拉帕米150μg/kg。休克组于相同时间输入等量生理盐水。结果显示:与休克组相比较,维拉帕米治疗能降低小肠组织黄嘌呤氧化酶活性和脂质过氧化产物含量,增加锰超氧化物歧化酶活性,减轻小肠组织线粒体和肌质损伤程度。实验结果提示维拉帕米能保护休克小肠的结构和功能,其作用机理与抑制氧自由基生成有关。
The role verapamil on the small intestine in canine hemorrhagic shock was studied.Anesthetized mongrel dogs were rapidly bled to a mean arterial pressure(MAP)of 6.0 KPa and maintained at that level for 90 min followed by resucitation with all shed blood.At 30min into hemorrhagic shock,verapamil(10μg/kg·min,n=6)or same volume of saline(3 ml/kg,n=5)was infused intravenously over 15 min,At the end of experiment(240 min after hemorrhage and resuscitation)the small intestine samples were obtained and analyzed.The results demonstrated that verapamil treatment significantly ameliorated intesine ultrastructure damage,exhibithd little malonyldialdehyde,low xanthine oxidase activity and high activity of Mn-SOD as compared with those of control dogs.These data suggest that verapamil has a beneficial effect on the intestines of dogs with hemorrhagic shock.
作者
欧阳静萍
李端祥
涂淑珍
杨海鹭
Ouyang Jingping;Li Duanxiang;Tu Shuzhen(Department of Pathophysiology,Hubei Medical University,Wuhan 430071,China)
关键词
维拉帕米
药理学
休克
出血性
小肠
verapamil/PD
shock,hemorrhagic
intesine,small/DE
dogs
animals,laboratory
