摘要
用三种方法测定血清免疫球蛋白(Ig)含量,并进行了彼此间差异的显著性和精密度的比较,认为三种方法测定血清Ig的含量无显著性差异(P>0.05),彼此间相关性较好(r=0.977)。而用免疫比浊法(ITM)测定血清Ig的精密度远高于单向免疫扩散法(RID)和火箭免疫电泳法(EIA)。用免疫比浊法测定IgG、IgA、IgM的正常参考值(X±s)分别为12.13±1.51g/L、2.04±0.55g/L、1.14±0.27g/L。慢性活动性肝炎IgG、IgA水平19.88±4.96g/L3.33±0.68/L与正常人比较差异有高度显著性(P<0.01);IgM水平1.26±0.24g/L与正常人比较无显著性差异(P>0.05).同时用免疫比浊法测定IgG、IgA、IgM有较好的回收率。
Three methods were used to detect serum immnoglobin and their precision and statistical significance compared.We thought there was not significant difference between these methods(P>0.05)and there was a high correlation(r=0.97).Precition of immunoturbidimetry(ITM)is far higher than that of radical immunodiffusion(RID)and rocket eletroimmunoassay(EIA).Normal reference values of ITM detecting IgG,IgA,IgM,were 12.13±1.51 g/L2.04±0.55 g/L1.14±0.27 g/L.Respectively IgG,IgA,level values of chronic active hepatitis were 19.88±4.96 g/L,and 3.33±0.68 g/L,and compared with the normal there was significant difference(P<0.01).IgM Value was 1.26±0.24 g/L,compared with normal there being not significant difference(P>0.05)in comparison with the normal.Moreover,there were good retrieval.
作者
夏林
李荷华
董新善
Xia Lin;Li Hehua;Dong Xinshan(Department of Clinical Labortory,Second Affiliated Hosipital,Hubei Medical University.Wuhan 430071,China)
关键词
免疫测定
免疫球蛋白类
血液
化学
immunoassay/MT
Immunoglobulins/BL
chemistry
clinical/MT
compartive study
