摘要
组织病理学判断胃癌标本淋巴结有无转移灶,对临床分期、治疗和预后具有重要价值。HE染色法较易识别淋巴结内成片的转移性癌细胞,但要发现微小转移灶较困难。对于那些播散到淋巴窦内的、形态酷似窦组织样细胞的癌细胞,或以淋巴瘤样形式浸润于淋巴网状组织中的癌细胞,仅以HE染色,则更难判断。为此,本研究应用胃癌单克隆抗体(McAb)免疫酶法,检测胃癌淋巴结转移,以探讨免疫组化法在提高胃癌淋巴结转移的检出率。材料与方法一、标本所有标本均系胃癌手术切除组织,福尔马林固定,石蜡包埋。
The histological detection of tumour metastases in lymph nodes from cases of gastric cancer is of major prognostic significance, but may be difficult when metastases are of microscopic size. We have, therefore, investigated whether the accuracy of metastases detection in lymph nodes can be increased by means of the monoclonal antibody immunocytochemical technique. 58 cases of gastric cancer were examined. The average number of lymph nodes examined per case being 3.5, with the total number ranging from 1 to 11. The histologically negative group comprises 29 cases. In 11 of these cases (37.93%), the immunocytochemical staining revealed involvement by tumor. The histologically positive group comprises 29 cases, in which lymph nodes metastases were reported in part, but not all, the immunostaining revealed 13 cases (28 lymph nodes) of the previously undetected metastases, which make up 44.83% of the positive group. These findings suggest that immunohistological assay may increase the positive rate of metastses detection in lymph nodes. In addition, there is a positive reaction to MG_9 in the mononuclear phagocytes of lymph node, which prognosticates that metastases may be earlier.
出处
《实用肿瘤杂志》
CAS
北大核心
1989年第1期23-25,共3页
Journal of Practical Oncology
