摘要
高纯度人绒毛膜促佐腺激素（HCG）的制备方法已有许多报道，通常都采用DEAE－纤维素离子交换层析法和磊胶过滤法反复槽制，价格昂贵．我们采用价格低廉的磷酸纤维赐作为寓子交换剂，宋代沓DEAE－纤维素，对具有历－20iu／mp生物效价的HCG粗品进行纯化，纯化后的HCG溶液，其紫外扫描图谱（220—300urn）与典型的蛋白质扫措图谱一致J经聚丙烯醉胺凝胶电泳分析，可得一条清楚的蛋白质条带；SDS－聚丙烯酞胺凝胶电泳分析，可见两条清晰的蛋白质色带．
Many methods have been reported for high purification of HCG. but those are usually purified with expensive DEAE-Cellulose. This paper presents a method to purify HCG using cheap cellulose phospho-rylated instead of DEAE-Cellulose. The chromatogram(220-300nm) of purified HCG is in conformity with the typical chromatogram of protein. The results show that HCG purified has one band in poly-acryla mide gel electrophoresis(PAGE) and two bands in SDS-PAGE.
出处
《杭州大学学报（自然科学版）》
CSCD
1994年第1期87-91,共5页
Journal of Hangzhou University Natural Science Edition
关键词
促性腺激素
磷酸纤维素
分离
HCG
HCG
cellulose Phosphorylated
ion-exchange chromatography
