摘要
本实验用聚合酶链反应（ＰＣＲ）技术对５６例受检者进行乙肝病毒（ＨＢＶ）ＤＮＡ检测，并将结果与血清标志物检测结果比较，结果表明，在ＨＢｓＡｇ、ＨＢｅＡｇ和抗ＨＢｃＡｇ同时阳性的受检者中，１００％可检测出ＨＢＶＤＮＡ。在血清标志物全阴性受检者中，也有４５％可测出ＨＢＶＤＮＡ。结合其他血清标志物的检测结果，说明用ＰＣＲ技术检测ＨＢＶＤＮＡ是敏感的，在临床应用上也是可行的。
Hepatitis B virus (HBV) DNA has been detected in the serum of 56 cases by the polymerase chain reaction (PCR)in this experimentand the results are compared with thoseobtained by serum marker determination.The daca have shown that HBV DNA could be foundat a rato of 100% in those who have positive reaction for HBsAg, HBeAg and anti-HBcAg.Inaddition,45% cases with negative serum marker also have detectable HBV DNA.Comparedwith other daca from serum maker determination,the resulto indicate tbat PCR technique is ofhigber sensitivity for determination of HBV DNA and may be used in future clinical practice.
出处
《海南大学学报（自然科学版）》
CAS
1994年第3期240-243,共4页
Natural Science Journal of Hainan University
关键词
聚合酶链反应
乙型肝炎
病毒
polymerase chain reaction
hepatitis B
virus
