摘要
莱姆病(Lyme disease)是最近十年来才被认识的一种蜱媒传染性流行病.莱姆病的血清学诊断方法主要有间接荧光免疫法(IFA)酶免疫试验(EIA),免疫印迹法(WB).梅迪埃(Biomerieux)公司推出免疫诊断系统(vidas).能够自动定性和半定量地检查Lyme病的抗伯氏包柔螺旋体抗体(抗BB抗体).本法结合了酶和荧光免疫二种方法.碱性磷酸酶标记二抗的底物是4-methylumbelliferylphosphate.底物在碱性磷酸酶的催化下转变成4—methylumbelliferon,该产物在365nm光激发下可发出455nm的荧光,光强度被Vidas中的光扫描器测定出来.我们用Vidas检测了体检标本83份.阳性8份(9.6%);检测临床为结核病的标本20份,无一例阳性;临床诊断结节病或可疑结节病标本46份,阳性4份(8.7%);各种眼科疾病57份,阳性13份82.8%);口腔科疾病15份.阳性4份(26.7%);其他如皮肌炎、肝脾肿大发热待查、肺癌、肺部 阴影待查等标本17份,阳性2份(11.8%);检测了军科院微生物流行病研究所采自新疆、黑龙江可疑Lyme病的标本16份,阳性12份(75.0%).作者用vidas法和IFA法比较了165份标本,vidas检出阳性24份(14.5%),IFA法检出131份(79.4%),两法的一致性(符合率)为32.7%.
Lyme disease is a infections disease just known in the latest decade, the sera from various origin people were detected for anti-BD antibodies(IgG+IgM) on VIDAS System. the results are as follows: of 83sera from the health-cared people, 8 were positive(the positive ratio 9. 6%); of 20 sera from the patients clinically diagnosed or doubted as node diseases, 4 were positive(8.75%); of 57 sera from the patients with variousophthalmologic diseasea. 4 were positive (26. 7%): of 17 sera from the patients with patients with various diseases(including dermomyositis, liver and spleen swelling, so on), 2 were positive(11.8%) tot 16 ser-a from the people highly doubled with Lyme province, 12 were positive(75%). At the same, we detected thesame 165 sera samples with VIDAS System and IRA, 24 were positive on VIDAS System(14. 5%)and 131 positive with IRA (79%), the accordance was onluy 32.7%.
