摘要
从四种田菁根瘤分离出４９Ｌ、５０Ｍ、５１Ｓ和５３号四株快生型根瘤菌。这些菌株能产酸，能利用的糖类较多，能在ｐＨ５．５～９．０和含盐（ＮａＣｌ）０．５％的培养基生长，最适的生长温度是２５～３０℃；５３号菌株还可以在ｐＨ１０和含盐１．０％的培养基于温度３５℃下生长。从毛萼田菁茎瘤分离出５４号慢生型菌株。此菌株能产碱，能利用的糖类较少，对ｐＨ适应幅度较小。这些菌株对宿主回接和互接均可结瘤固氮。田间种植的田菁根瘤有较高的固氮酶活性和氢酶活性。毛萼田菁的根瘤和茎瘤这两种酶活性更高。田菁根瘤菌抗逆性强。其寄主田菁是改良盐碱地的优良植物，毛萼田菁更好。
The rhizobial strains 49L, 50M and 51S, as well as 53 from the root nodules of four sesbania specieshave been isolated. They are fast-growing, which produce acid on yeast-mannitol agar and utilize most of carbohydrates as carbon Source. The optimum growth temperature is 25～30℃, grow well at pH 5. 5～9. 0 andtolerate to 0. 5% NaCl. Strain 53 can also grow vigorously at 35℃ and PH 10, tolerate to 1. 0% NaCl. Aslow-growing strain 54 from stem nodule of Sesbania rostrata has been isolated. On yeast-mannitol agar, it produces alkali substance. The pH range for its growth is 6～8, optimum is 7. 0. It grows well at 25～30℃, tolerates to 1. 0% NaCl and utilizes less carbohydrates. When inoculating or cross-inoculating, the host plantsformed nodules with high nitrogenase and hydrogenase activities.
基金
国家自然科学基金
关键词
田菁
根瘤
根瘤菌
特性
分离
Sesbania
Nodules
Rhizobial strain
Characterization
