摘要
对周毛德克斯氏菌胞外多糖提取、纯化方法的研究表明该多糖最佳培养时间为３０±２ｈ，粘度为６．７７－９．２７Ｐａ·ｓ，离心可除去菌体，添加质量分数为１×１０－２的ＮａＣｌ可减少沉淀多糖所需的乙醇用量３／５．经高效液相色谱法对该胞外多糖组分分析，结果表明其由鼠李糖、木糖、葡萄糖、甘露糖、阿拉伯糖、果糖和两种未知成分组成．
The studies on the method of extraction and purification of the extracellular polysaccharide from Derxia peritricha revealed that the optimum cultural duration is 30±2 hours for producing the polysaccharide. The biomass can be removed by means of centrifugation in the condition of the viscosity between 6.77-9.27 Pa·s. The amount of alcohol used for purification can be decreased 3/5 with adding 1×10-2 NaCl. The result of component analysis by the method of HPLC demonstrated that this extracellular polysaccharide contains rhamnose, xylose, glucose, mannose, arabinose, fructose and two other unidentified monosaccharides.
出处
《福建农业大学学报》
CSCD
1994年第4期476-479,共4页
Journal of Fujian Agricultural University
基金
福建省自然科学基金
关键词
周毛德克斯氏菌
胞外多糖
化学组分
Derxia peritricha
extracellular polysaccharide
chemical componet
