摘要
目的：对甲型肝炎（甲肝）患者血清ＩｇＭ及ＩｇＧ型抗ＨＡＶ－抗独特型抗体（抗ＨＡＶ－抗Ｉｄ）进行动态观察，探讨其在甲肝发病机理中的可能作用．方法：在Ｉｒｓｈａｄ等方法的基础上建立了抗ＨＡＶ－抗Ｉｄ的ＥＬＩＳＡ法．结果：抗ＨＡＶ－抗Ｉｄ在甲肝急性期以ＩｇＭ型为主（３０．１２％），ＩｇＧ型仅少量出现（８．４３％）；恢复期以ＩｇＧ型为主（３１．３３％，Ｐ＜０．０１），ＩｇＭ型则明显减少（１５．６６％，Ｐ＜０．０５），与甲肝抗体在急性期以ＩｇＭ型为主，恢复期以ＩｇＧ型为主相符．恢复后期虽然抗ＨＡＶ抗体大都存在，但抗ＨＡＶ－抗Ｉｄ却大部分转阴．结论：抗ＨＡＶ－抗Ｉｄ的出现与甲肝的病理过程相符，均为一过性；ＨＡＶ病毒血症时间很短，但甲肝抗体却能长期存在，可能与抗ＨＡＶ－抗Ｉｄ发挥ＨＡＶ“内影像”作用有关．
Objective:To observe the dynamic changes of IgM and IgG anti-idiotypic antibodies against anti-HAAg(anti- Id)in the sera of hepatitis A(HA)patients,in order to reveal anti-Id’s possible affect which act on the pathogenic mechanism of HA.Methods:Using the ELISA for anti-Id established on the basis of Irshad’s method.Results:In acute stage of HA,anti-Id was mainly IgM ones(30.12%)and possessed of a few IgG ones(8.4%);in convalescence,there were a great part of IgG ones(31. 33%,P<0.01), and IgM antibodies decreased significantly(15.66%,P<0.05 ). The result is in accordance with that there were mainly IgM anti-HAAg in acute stage and mainly IgG ones in convalescence. In the later stage of convales-cence,though most IgM and IgG anti-HAAg existed in sera,most of the anti-Id had become negative. Con-clusions:1.The presence of anti-Id in sera of HA patients was a transient change, which is corresponding to the HA pathological course; 2.The persisting existence of anti-HAAg,though the virusemia of HAV was a quite short course,might be related to the anti-Id’s internal image effect functioned in HAV.
出处
《第四军医大学学报》
1994年第2期122-124,共3页
Journal of the Fourth Military Medical University
关键词
甲型肝炎
免疫球蛋白M
免疫球蛋白G
hepatitis A
antibodies anti- idiotypic
immunoglobulin M
immunoglobulin G
enzyme-linked immunosorbent assay
