摘要
作者应用小鼠抗肾综合征出血热病毒（ＨＦＲＳＶ）单克隆抗体（ＭＡｂｓ）对经检测血清抗体阳性率为２８％的５０只大鼠组织中病毒抗原及抗体（内源性免疫球蛋白，ＥＩｇ）进行了研究。结果：５０只大鼠中，１５只仅检测到病毒抗原阳性，１０只仅检测到ＥＩｇ阳性，１８只抗原、ＥＩｇ均阳性，７只抗原、ＥＩｇ均阴性，其中的血清抗体阳性鼠分别有２，６，３，３只。总抗原阳性率为６６％，ＥＩｇ阳性率为５６％。病毒抗原分布于心肌细胞、肝细胞、脾淋巴细胞、肺支气管粘膜上皮及肺泡壁、肾小管上皮细胞、唾液腺闰管及腺上皮，以及肾上腺腺细胞，Ｇ２抗原多为弥漫性，ＮＰ抗原呈颗粒状包涵体或弥漫性阳性，其中１８只组织中可同时有抗体阳性。ＥＩｇ阳性见于各组织的血管内皮，特别是肾小球毛细血管，以及单核吞噬细胞系统；在心肌和肝脏有呈散在分布的心肌及肝细胞ＥＩｇ阳性。以上结果提示：血清抗体，组织中抗原和抗体三者间无明确一致关系，抗原的检测可能更好地反映动物的带毒状况；另外，大鼠组织中有抗原和免疫复合物存在，作者在本文对大鼠感染ＨＦＲＳＶ病变不严重，亦不引起发病进行了讨论。
group of laboratory-bred Wistar rats was confirmed to be naturally infected with hemorrhagicfever with renal syndrome virus(HFRSV)by the serum antibody detection using Sandiwich ELISA method.Fifty rats,of which the anti-HFRSV antibody positive rate was 28%,were sacrificed,the viral antigens andantibody(endogenous immunoglubulin, EIg)in the tissues were detected by a novel immunocytochemicalmethod recently developed in our Lab.which could be used for the demonstration of the specific binding ofmouse monoclonal antibodies(MAb)to the mouse or rat tissues. The relationship of the viral antigens andEIg in the tissues was further appreciated.The results showed that, of 50 rats,15 rats were viral antigenspositive only; 10 rats EIg positive only; 18 rats both the antigens and EIg positive and other 7 rats both nega-tive; the serum anti-HFRSV antibody positive rats were distributed among those rats described above respec-tively. In the antigen positive rats,the glycoprotein Ⅱof HFRSV(G2)was demonstrated to be mainly indiffuse staining pattern, and the nucleocapsid protein(NP)in the form of vesicles or coarse granular inclu-sion body or in diffuse staining pattern,the antigens were widely distributed in the tissues and cells such asmyocardial cells,hepatocytes,epithelial cells of bronchial mucosa,renal tubules and siliva glands andparenchymal cells of adrenal glands. In 18 of 33 viral antigen positive rats and 10 viral antigen negative rats,the EIg was positive and localized in the vasculatures espically in the glomerular tufts and monocyte-macrophage system,on the other hand ,the EIg positive myocardial cells and hepatocytes in dispersive distri-bution were also observed. In the antigen and/or EIg positive tissues,there were no evidences such as tissueedema,hemorrhage,inflammation or necrosis could suggests the abnormal vascular permeabilities and the damages due to the antigens and EIg or immunocomplex. The results indicated that :①the viral antigen de-tection were more practical for the epidemiological investigation of animal infection than the serum antibodydetection only;②the virus direct attack only or the deposition of the large molecular immune complexes onthe vasculatures had the less abilities to induce the abnormal permeabilities of the cellular membranes andcapillaries and make the rats illness;3.other mechanisms such as cell-mediated immunity might be involvedin the eradication of the virus from the host cells and the cellular membranous lesions.
出处
《第四军医大学学报》
1994年第1期49-53,共5页
Journal of the Fourth Military Medical University
关键词
肾综合征
出血热
病毒
抗原
抗体
hemorrhagic fever with renal syndrome
viruses
antigens
antibodies
rats
infection
natural
immunohistochemistry
