摘要
Ｗｉｓｔａｒ大鼠经６Ｇｙ６０Ｃｏγ线和／或３０％总体表面积（ＴＢＳＡ）Ⅲ度烧伤致放射损伤（ＲＩ）、烧伤（ＢＩ）和放烧复合伤（ＲＢＩ）．观测伤后１、３、８、１６和２４ｈ大鼠心肌线粒体脂质过氧化和抗氧化酶活力的改变，发现三种伤类均表现为脂质过氧化代谢产物丙二醛（ＭＤＡ）含量伤后１ｈ即明显升高，２４ｈ降至对照组（Ｃ）水平；超氧化物歧化酶（ＳＯＤ）活力在整个实验过程中维持在明显低于Ｃ组的水平；ＢＩ组和ＲＢＩ组还可见谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活力在伤后１６和２４ｈ明显降低。结果表明，伤后心肌线粒体抗氧化酶活力降低，脂质过氧化加强，提示线粒体内有自由基堆积。结合此前同样改变条件下所见的心功能下降，认为自由基的有害作用可能是心功能降低的重要原因之一。
The content of malondialdehvde(MDA)and the activity of antioxidization enzyrnes of the myocardial mitochondria were observed in rats,which were randomized into the control(C),burn injury(BI),radiation injury(RI)and combined radiation-burn injury(CRBI)groups,in the lst,3rd,8th,16th and 24th hour after injury,The findings were as follows;1,The MDA content was increased significantly in the lst hour and returned gradually to the control level in the 24th hour,The activity of superoxide dismutase(SOD)was kept in an obviously lower level than that of the control throughout the observation period in BI,RI and CRBI groups.2.The activity of GSH-Px was decreased in BI and CRB groups.The fidings indicate that the capacity to scavenge free radicals is weakened and lipid peroxidation intensified in the,myocardial mitochondria of the injured rats,Consequently,it is deduced that the harmful effects of free radicals might be one of the factors to induce cardiac dysfunction,injury under the same injurious in our previous report.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1994年第6期430-433,共4页
Journal of Third Military Medical University
关键词
灼伤
放射损伤
复合伤
过氧化脂质
烧伤
burns
radiation injuries,experimental
combined radiation-burn injury
lipid peroxides
mitochondria,heart
rat
