摘要
应用多聚酶链延伸反应──限制性片段长度多态性分析法（ＰＣＲ－ＲＦＬＰ）对４３例福尔马林固定、石蜡包埋肝癌组织癌基因ｒａｓ和抑癌基因Ｐ^（５３）点突变进行分析。结果表明，ｒａｓ基因总突变率为４１．９（１８／４３），其中Ｎ－ｒａｓ第１２位密码子突变率为３７．２％（１６／４３），ｃ－Ｋｉ－ｒａｓ第１２位密码子突变率为６．９％（３／４３），ｃ－Ｋｉ－ｒａｓ第１３位、ｃ－Ｈａ－ｒａｓ第１２和６１位密码子未发现有突变者。Ｐ^（５３）基因第２４９密码子突变率为２０．９％（９／４３），第２４８密码子未发现有突变者。
The point mutation of ras oncogenes and suprressor gene P^(53) were studied in 43 formalin-fixed and paraffin embedded specimens of primary hepatocellular carcinoma with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). It was found that the overall rate of the point mutation of ras genes was 41.9% (18/43). Mutation at codon 12 of N-ras and codon 12 of c-Ki-ras were identifiedin 37.2%(16/43) and 6.9%(3/43)of cases but no mutation could be detected at codon 13 of c-Ki-ras and codon 12 and 61 of c-Ha-ras. Mutation at codon 249 of P￣(53)was found in 20.9%(9/43)of cases but none at codon 248.These findings suggest that the point mutation at codon 12 of N-ras and codon 249 of P￣(53) is common in hepatocellular carcinoma.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1994年第5期340-343,共4页
Journal of Third Military Medical University
