摘要
从蚯蚓中分离得到的能够抑制离体豚鼠心耳收缩的活性成分,经放射性配体结合实验表明,能够抑制~8H标记的心得舒与鸭红细胞膜制剂内β—肾上腺素能受体的结合。在腺苷酸环化酶活性测定中能抑制异丙基肾上腺素对酶的激活作用。为一种新的内源性的β—肾上腺素能受体阻滞剂。
The biochemical and pharmacologic characteristics of earthworm inhibitory preparation of polypeptide nature were investigated. This endogenous substance can competitively inhibit 3H-DHA binding to β-receptor on duck erythrocyte membranes and decrease the Bmax on the same membranes obviously. In addition, it can inhibit adenylate cyclase (AC) activity stimulated by isoproterenol(ISO) . The experiments showed that The earthworm inhibitory preparation is not only a kind of Ligand of β-receptor, but also a novel β-adrenergic receptor antagonist of polypeptide nature
关键词
蚯蚓抑制剂
肾上腺素能
受体阴滞剂
earthworm inhibitory preparation Ligand-receptor binding AC activity β-adrenergic receptor antagonist
