摘要
将含昆虫血淋巴、鸡蛋黄和牛奶的培养液用帕拉玢膜加工成小囊状“人工幼虫”,将寄生在大蜡螟幼虫体表接近孵化的麦蛾茧蜂卵,移到“人工幼虫”上培养。麦蛾茧蜂完成从卵到成虫离体发育,幼虫存活率、化蛹率和成虫羽化率分别为72％、48％和24％。
Mature eggs of H. hebetor were collected from parasitized larvae of greater wax moth, Galleria mellonella in the laboratory, and transferred onto the sac-like 'artificial larvae' for the in vitro culture. The 'artificial larvae' were made of artificial diets composed of oak silkworm pupal hemqlymph, egg yolk and dried milk suspension, in various ratios, and encapsulated with parafilmR. The parasite completed its development from egg to the adult stage on the 'artificial larvae' in 12-13 days under 26-28℃ and 75-85% RH. The survival rate of larvae, pupae and adults were 72%, 48% and 24%, respectively.
关键词
麦蛾茧蜂
离体培养
生物防治
Habrobracon hebetor in vitro rearing parasitoid
